6/1/2023 0 Comments Schistosome fluke worm in ca![]() ![]() The resulting digestion pattern differed from that brought about by kallikrein, a host serine protease that normally acts on HK to release the vasodilator bradykinin. ![]() Incubating parasites in buffer with pure HK followed by Western blot analysis confirmed that human HK is degraded by the worms. The most striking change to the plasma proteome concerned HK while the full-length protein was more abundant in control plasma, carboxyl-terminal truncated forms were more abundant in plasma that contained schistosomes. Finally, the generation of bradykinin from HK was monitored using a bradykinin detection kit. A major change to the blood protein kininogen (HK) was observed, and the interaction of Schistosoma mansoni parasite with this protein alone was then investigated by western blot analysis and activity assays. Methodsįollowing the incubation of adult schistosomes in murine plasma, two-dimensional differential in-gel electrophoresis (2D-DIGE) was conducted to look for changes in the plasma proteome compared with control plasma. Here we examined the influence of the parasites on their external environment by monitoring the impact of adult Schistosoma mansoni worms on the murine plasma proteome in vitro and, in particular, on how the worms affect the blood coagulation protein high molecular weight kininogen (HK). Schistosomes are blood dwelling parasitic worms that cause the debilitating disease schistosomiasis.
0 Comments
Leave a Reply. |